Detecção do DNA dos papilomavírus tipos 16 e 18 em lesões epiteliais adquiridas da conjuntiva

Detecção do DNA dos papilomavírus tipos 16 e 18 em lesões epiteliais adquiridas da conjuntiva


Sung Bok Cha,
Jerry A. Shields,
Ralph Eagle Jr.,
Ming Wang,
Marinho J. Scarpi


Arquivos Brasileiros de Oftalmologia

versão impressa ISSN 0004-2749versão On-line ISSN 1678-2925

Arq. Bras. Oftalmol. vol.60 no.2 São Paulo abr. 1997


The occurrence of certain types of HPV DNA, such as 16 and 18, in epithelial lesions of the ocular conjunctiva was analysed in this present study.

We studied 31 samples of epithelial lesions which the pathological diagnosis varied from metaplasia to invasive squamous cell carcinoma of the conjuntiva, embedded in paraffin blocks.

The samples was divided in two groups: 1) tissue samples from patients who developed recurrence after a former surgery and 2) tissue samples from patients who never developed recurrence. The detection of HPV DNA was done by PCR and "dot blot" hybridization techniques.

A positivity of 65% to HPV DNA was found in the group with recurrence and 65% in the group without recurrence. With regard to HPV DNA type 18, we found a positivity of 9% and 5% in the groups with and without recurrence, in this arder.

The incidence of HPV DNA types 16 and 18 was relatively high. This result could suggest a possibl envolvement of HP V in the pathogenesis of epithelial tumors such as squamous cell carcinoma of the conjunctiva.


1 TABBARA, K.; KERSTEN, R.; DAOUK, N.; B DI, F. - Metastatic squamous cell carcinoma 01 conjuntiva. Ophthalmology 95: 318-21, 1988.
2 HOWLEY, P. - Papillomavirinae and their repli cation. In: FIELDS, B.; KNIPE, D. - Fundamental Virology 2ª ed., Raven Press Ltd., New York, p. 743-68, 1991.
3 BROKER, T. - Structure and genetic expression of papillomaviruses. Obstet Gynecol Clin North Am 14: 329-48, 1987.
4 McDONNELL, J.; McDONNELL, P.; SUN, Y. - Human papillomavirus DNA in tissues and ocu lar surface swabs of patients with conjunctival epithelial neoplasia. Invest Ophthalmol Vis Sci 33: 184-9, 1992.
5 PFISTER, H. - Biology and biochemistry of papillomaviruses. Rev Physiol Biochem Phar macol 99: 111-81, 1984.
6 SILLMAN, F. & SEDLIS, A. - Anogenital papillomavirus infection and neoplasia in immunodeficient women. Obstei Gynecol Clin North Am 14: 537-58, 1987.
7 BRESCIA, R.; JENSON, A.; LANCASTER, W. - The role of human papillomaviruses in the pathogenesis and histologic classification of precancerous lesions of the cervix. Hum Pathol 17: 552-9, 1986.
8 CRUESS, A.; WASAN, S.; WILLIS, W. - Corneal epithelial dysplasia and carcinoma in situCan J Ophthalmol 16: 171-5, 1981.
9 SHIBATA, D.; ARNHEIM, N.; MARTIN, J. - Detection of human papillomavirus in paraffin- embedded tissue using the polymerase chain reaction. J Exp Med 167: 225-30, 1988.
10 SAIKI, R.; GELFAND, D.; STOFFEL, S.; SCHARF, S.; HIGUCHI, R.; HORN, G.; MUL- LIS, K.; ERLICH, H. - Primer-directed enzyma tic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-91, 1988.
11 DAVIS, L.; DIBNER, M.; BATTEY, J. - "Dot Blot" hybridization of labeled probe to DNA or RNA samples 1ª ed., New York, Elsevier, p. 147-9, 1986.
12 GROSSNIKLAUS, H.; GREEN, W.; LUCKEN BACH, M.; CHAN, C. - Conjuntival lesions in adults. A clinical and histopathologic review. Cornea 7: 79-86, 1987.
13 ASH, J. - Epibulbar tumors. Am J Ophthalmol33: 1203-19, 1950.
14 MAUMENEE, A. - Keratinization of the conjuctiva. Trans Am Ophthalmol Soc 77: 133-43, 1979.
15 McDONNELL, J.; MAYR, A.; MARTIN, W. - DNA of human papillomavirus type 16 in dysplasic and malignant lesions of the conjunctiva and cornea. N Engl J Med 320: 1442-6, 1989.
16 SANJOSÉ, S.; SANTAMARIA, M.; RUIZ, P.; ARISTIZABEL, N.; GUERRERO, E.; CASTE- LLSAGUÉ, X.; BOSCH, F. - HPV types in women with normal cervical cytology. ln: MUNÖZ, N.; BOSCH, F.; SHAH, K.; ME-HEUS, A. - The epidemiology of human papillomavirus and cervical cancer. Lyon, IARC Sci. Publ. 119: 135-45, 1992.
17 LASS, J.; JENSON, A.; PAPALE, J.; ALBERT, D. - Papillomavirus in human conjunctival papillomas. Am J Ophthalmol 95: 364-8, 1983.
18 FRAUNFELDER, F. & WINGFIELD, D. - Management of intraepithelial conjunctival tumors and squamous cell carcinomas. Am J Ophthalmol 95: 337-49, 1983.
19 de VILLIERS, E.; WAGNER, D.; SCHNEIDER, A.; WESCH, H.; MUNZ, F.; MIKLAW, H.; zur HAUSEN, H. - Human papillomavirus DNA in women without and with cytological abnor malities: Results of a five-year follow-up study. Gynecol Oncol 44: 33-9, 1992.
20 CROOK, T.; WREDE, D.; TIDY, J.; SCHOLE- FIELD, J.; CRAWFORD, L.; VOUSDEN, K. Status of c-myc, p53 and retinoblastoma genes in human papillomavirus positive and negative Sqamous Cell Carcinoma of The anus. Onco gene 6: 1251-7, 1991.