Envolvimento da proteína A purificada e do Staphylococcus aureus na patogênese corneana

Envolvimento da proteína A purificada e do Staphylococcus aureus na patogênese corneana


Ginaine Farjallah Bazzi,
Fabio Prado Sabbag,
Marcelo Luiz Gehlen,
Pablo Fabian Aviles Cabrera,
Luciane Bugmann Moreira,
Cinthia Oyama Branco,
João Carlos Domingues Repka,
Hamilton Moreira


Arquivos Brasileiros de Oftalmologia

versão impressa ISSN 0004-2749versão On-line ISSN 1678-2925

Arq. Bras. Oftalmol. vol.60 no.3 São Paulo jun. 1997


Myeloperoxidade activity in experimental keratitis (Guinea Pigs) was studied. Staphylococcus aureus strain DU 5723 without Protein A nor Delta-toxin (Group A), purified Protein A (SIGMA) (Group B), and vehicle were injected within corneal stroma, with a final volume of 10 µl. Levels of myeloperoxidase activity after 25 hours were measured by spectrophotometric method and the tissue damage was determined by biomicroscopic analysis. These results suggest that purified Protein A and S. aureus without Protein A nor Delta-toxin induces high leveis of tissue damage and both inocula may be mediators of keratitis.


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