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Influência do epitélio corneal limbar sobre a expressão do antígeno dos linfócitos da mucosa humana (HML-1)

Influência do epitélio corneal limbar sobre a expressão do antígeno dos linfócitos da mucosa humana (HML-1)

Autores:

José Alvaro Pereira Gomes,
Luiz Rizzo,
Mauro Nishi,
Larry A. Donoso,
Harminder Singh Dua

ARTIGO ORIGINAL

Arquivos Brasileiros de Oftalmologia

versão impressa ISSN 0004-2749versão On-line ISSN 1678-2925

Arq. Bras. Oftalmol. vol.60 no.6 São Paulo dez. 1997

http://dx.doi.org/10.5935/0004-2749.19970006

SUMMARY

The intraepithelial lymphocytes (IELs) may play an important role in the defense and tolerance induced by the mucosal associated lymphoid tissue (MALT). In the mucosal epithelium, IELs express the human mucosal lymphocyte integrin (HML-1), which is important in their adherence to the epithelial cells. In this experiment, the authors investigate the mechanisms of HML-1 expression on peripheral blood lymphocytes associated with corneal limbal epithelium.

Lymphocitic and corneal epithelial cultures were performed. Three groups were determined: Group A, non-activated lymphocytes with corneal limbal epithelial cells in culture media; Group B, nonactivated lymphocytes separated by a semi-permeable membrane from epithelial cells in culture media; and group C, non-activated lymphocytes in culture media (control). In the seventh day, the lymphocytes were recovered and sent to jlow citometric analysis of the antigens CDS/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25 and CD4/CD25.

Comparisons amongst groups A, B and C were dane by the repeated-measured statistical anaiysis (ANOVA) and Student's paired -t test.

Statistically significant differences were observed in the following lymphocytic sub-populations: CD8/HML-1 + (p=0,001), CD3/CD8+ (p=0,006), CD3/CD25+ (p=0,023) e CD8/CD25+ (p=0,002).

This experimental study suggests that the HML-1 expression by the CD8+ peripheral T lymphocytes increase may be dependent upon direct and indirect interactions with corneal epithelium. Growth factors and cytokines may play a role in HML-1 expression.

REFERÊNCIAS

1 STROBER, W. & JAMES, S. P. - The mucosal immunesystem. In: Stites, D. P. & Terr, A. I., eds. Basic and clinical immunology. 7th ed. Norwalk: Appleton and Lange; 175-186, 1991.
2 DUA, H. S.; GOMES, J. A. P.; JINDAL, V. K.; APPA, S. N.; SCHWARTING, R.; EAGLE JR., R. C.; LAIBSON, P. R. - Mucosa specific lymphocytes in the human conjunctiva, corneoscleral limbus and lacrimal gland. Curr. Eye Res., 13: 87-93, 1994.
3 CERF-BENSUSSAN, N. & GUY-GRAND, D. - Intestinal intraepithelial lymphocytes. Gastroenterol. Clin. North Am., 20: 549-76, 1991.
4 TRIER, J. S. - Structure and function of intestinal M cells. - Gastroenterol. Clin. North Am., 20: 531-547, 1991.
5 WERSHJL, B. K. & GALLI, S. J. - Gastrointestinal mast cells: new approaches for analysing their function in vivo. Gastroenterol. Clin. North Am., 21: 613-27, 1991.
6 NUSSENBLATT, R. B.; CASPI, R. R.; MAHDI, R.; CHAN, C.C.; ROBERGE, F.; LIDER, 0.; WEINER, H. L. - Inhibition of S-antigen induced experimental autoimmune uveoretinitis by oral induction of tolerance with S-antigen. J. lmmunol., 144: 1689-1695, 1990.
7 DUA, H. S.; DONOSO, L. A.; LAIBSON, P. R. - Conjunctival instillation of retinal antigens induces tolerance. Does it invoke mucosal tolerance mediated via conjunctival associated lymphoid tissue (CALT)? Ocular lmmunol. Inflam., 2: 29-36, 1994.
8 PARKER, C. M.; CEPEK, K. L.; RUSELL, G. J.; SHAW, S. K.; POSNETT, D. N.; SCHWARTING, R.; BRENNER, M. B. - A family of 137 integrins on human mucosal lymphocytes. Proc. Natl. Aead. Sci. USA., 89: 1924-1928, 1992.
9 CERF-BENSUSSAN, N.; JARRY, A.; BROUSSE, N.; LISOWSKA-GROEPIERRE, B.; GUY-GRAND, D.; GRISCELLI, C. - A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur. J. Immunol., 17: 1279-1285, 1987.
10 SHIEFERDECKER, H. L.; ULLRICH, R.; WEISS-BRECKWOLDT, R.; SCHWARTING, R.; STEIN, H.; RIECKEN, E.; ZEITZ, M. - The HML-1 antigen of intestinal lymphocytes is an activation antigen. J. Immunol., 144: 2541-2549, 1990.
11 CEPEK, K. L.; SHAW, S. K.; PARKER, C. M.; RUSSELL, G. J.; MORROW, J. S.; RIMM, D. L.; BRENNER, M. B. - Adhesion between epithelial cells and T lymphocytes mediated by Ecadherin and the aEβ7 integrin. Nature., 372: 190-193, 1994.
12 FRANKLIN, R. M. & REMUS, L. E. - Conjunctival-associated lymphoid tissue: Evidence for a role in the secretory immune system. Invest. Ophthalmol. Vis. Sci., 25: 181-187, 1984.
13 DUA, H. S.; GOMES, J. A. P.; DONOSO, L. A.; LAIBSON, P. R. - The ocular surface as part of the mucosal immune system: conjunctival mucosa-specific lymphocytes in ocular surface pathology. Eye., 9: 261-267, 1995.
14 EBATO, B.; FRIEND, J.; THOFT, R. A. Comparison of central and peripheral human corneal epithelium in tissue culture. Invest. Ophthalmol. Vis. Sci., 28: 1450-1456, 1987.
15 FOTINO, M.; MERSON, E. J.; ALLEN, F. H. Micromethod for rapid separation of lymphocytes from peripheral blood. Ann. Clin. Lab. Sci., 1: 131-133, 1971.
16 SPRINGER, T. A. - Adhesion receptors of the immune system. Nature., 346: 425-434, 1990.
17 IWATA, M.; KIRITOSHI. A.; ROAT, M. I.; YAGIHASHI, A.; THOFT, R. A. - Regulation of HLA Class II antigen expression on cultured corneal epithelium by interferon-gama. Invest. Ophthalmol. Vis. Sci., 33: 2714-2721, 1992.
18 SHAMS, N. B. K.; HUGGINS, E. M.; SIGEL, M. M. - Interleukin-1 regulates the proliferation of leukocytes in human corneal cell-peripheral blood leukocyte cocultures. Cornea., 13: 9-15, 1994.
19 CHEN, W. Y. W.; MUI, M. M.; KAO, W. W. Y.; LIU, C. Y.; TSENG, S. C. G. - Conjunctival epithelial cells do not transdifferentiate in organotypic cultures: expression of K12 keratin is restricted to corneal epithelium. Curr. Eye Res., 13: 765-78, 1994.
20 GOMES, J. A. P.; RIZZO, L.; NISHI, M.; DONOSO, L. A.; DUA, H. S. - Human mucosal lymphocyte antigen (HML-1) expression on lymphocytes induced by corneal and conjunctival epithelial cells. ARVO Abstracts. Invest. Ophthalmol. Vis. Sci., 37: Sl078, 1996.
21 SPORN, M. B. & ROBERTS, A. B. - Autocrine secretion- 10 years later. Ann. Rev. Cell Biol., 4: 229-255, 1988.
22 WILSON, S. E.; SCHULTZ, G. S.; CHEGINI, N.; WENG, J.; HE, Y. G. - Epiderrnal growth factor, transforrning growth factor beta, acidic fibroblast growth factor, basic fibroblast growth factor and interleukin-1 proteins in the comea. Exp. Eye Res., 59: 63-72, 1994.