O valor da análise bioquímica de Staphylococcus aureus na determinação da sensibilidade à eritromicina

Autores:

Alexandre S. Marcon,

Giovanni M. Travi,

Italo M. Marcon,

Zélia M. Corrêa

ARTIGO ORIGINAL

Arquivos Brasileiros de Oftalmologia

versão impressa ISSN 0004-2749versão On-line ISSN 1678-2925

Arq. Bras. Oftalmol. vol.62 no.2 São Paulo abr. 1999

http://dx.doi.org/10.5935/0004-2749.19990031

SUMMARY

Purpose:

To assess the reliability of biochemical pattern (biotype) to determine erythromycin sensitivity of multiple Staphylococcus aureus isolates from the conjunctiva and lid margin of healthy eyes.

Methods:

The authors analyzed 42 samples of S. aureus, isolated from 100 volunteers, through MicroScan® (Dade lnternational, West Sacramento, CA) Pos-Combo type 6 panels. Biochemical reactions were organized in a table. The biotype number was determined by adding values of each reaction according to the manufacturer's instructions.

Results:

Biotype 317147 was more prevalent (n = 19), 11 being sensitive to erythromycin and 8 resistant. Other biotypes were analyzed: 317163 (n = 8), 317167 (n = 6), 317347 (n = 2), etc.

Conclusion:

It was concluded that biotyping alone can not predict everythromycin sensitivity erythromycin sensibility for S. aureus biotype 317147 (CI 95%o, 0.29-0.087 for sensible isolates and 0.08-0.76 for resistant isolates).

Keywords: Staphylococcus aureus; MicroScan; Erythromycin; Biotyping

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